翻訳と辞書 Words near each other ・ Multiple cloning site ・ Multiple comparisons problem ・ Multiple complex developmental disorder ・ Multiple component pricing ・ Multiple Console Time Sharing System ・ Multiple correlation ・ Multiple correspondence analysis ・ Multiple cropping ・ Multiple cutaneous leiomyoma ・ Multiple deprivation index ・ Multiple description coding ・ Multiple disabilities ・ Multiple discovery ・ Multiple discriminant analysis ・ Multiple dispatch ・ Multiple displacement amplification ・ Multiple document interface ・ Multiple drafts model ・ Multiple drug resistance ・ Multiple edges ・ Multiple EM for Motif Elicitation ・ Multiple encryption ・ Multiple endocrine neoplasia ・ Multiple endocrine neoplasia type 1 ・ Multiple endocrine neoplasia type 2 ・ Multiple endocrine neoplasia type 2b ・ Multiple Epidermal Growth Factor-like Domains 8 ・ Multiple epiphyseal dysplasia ・ Multiple Equivalent Simultaneous Offers ・ Multiple evanescent white dot syndrome Dictionary Lists mini英和辞書 mini和英辞書 Webster 1913 Latin-English FOLDOC Wikipedia English ウィキペディア

翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Multiple displacement amplification ： ウィキペディア英語版 Multiple displacement amplification Multiple displacement amplification (MDA) is a non-PCR based DNA amplification technique. This method can rapidly amplify minute amounts of DNA samples to a reasonable quantity for genomic analysis. The reaction starts by annealing random hexamer primers to the template: DNA synthesis is carried out by a high fidelity enzyme, preferentially Φ29 DNA polymerase, at a constant temperature. Compared with conventional PCR amplification techniques, MDA generates larger sized products with a lower error frequency. This method has been actively used in whole genome amplification (WGA) and is a promising method for application to single cell genome sequencing and sequencing-based genetic studies. == Background == Many biological and forensic cases involving genetic analysis require sequencing of DNA from minute amounts of sample, such as DNA from uncultured single cells or trace amounts of tissue collected from crime scenes. Conventional Polymerase Chain Reaction (PCR)-based DNA amplification methods require sequence-specific oligonucleotide primers and heat-stable (usually ''Taq'') polymerase, and can be used to generate significant amounts of DNA from minute amounts of DNA. However, this is not sufficient for modern techniques which use sequencing-based DNA analysis. Therefore, a more efficient non-sequence-specific method to amplify minute amounts of DNA is necessary, especially in single cell genomic studies. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Multiple displacement amplification」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.